GFP anticorps

N° du produit ABIN966200

L’anticorps Souris Monoclonal anti-GFP a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter GFP dans des échantillons de Aequorea victoria. Il y a 2+ publications disponibles.

Aperçu rapide pour GFP anticorps (ABIN966200)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 4B10B2

Détail du produit anti-GFP anticorps

Purification: Purified

Immunogène: Purified recombinant fragment of GFP expressed in E. Coli.

Isotype: IgG2a

Information d'application

Indications d'application: Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Buffer: PBS

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-GFP (ABIN966200)

Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, (2006) (PubMed).

Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." dans: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, (2006) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Poids moléculaire: 27kDa